Open AccessThe potential effects of green tea (-)-epigallocatechin-3-gallate on overcoming imatinib-resistance in chronic myeloid leukemia bearing BCR-ABL
Author(s) -
Bùi Thị Kim Lý,
Hoàng Thanh
Publication year - 2019
Publication title -
tạp chí khoa học đại học sư phạm thành phố hồ chí minh
Language(s) - English
Resource type - Journals
ISSN - 2734-9918
DOI - 10.54607/hcmue.js.14.9.2207(2017
Subject(s) - myeloid leukemia , imatinib , imatinib mesylate , apoptosis , chemistry , abl , protein kinase b , cancer research , mapk/erk pathway , k562 cells , microbiology and biotechnology , kinase , pharmacology , biology , signal transduction , tyrosine kinase , biochemistry
We investigated the effect of (-)-epigallocatechin-3-gallate (EGCG) in overcoming imatinib mesylate -resistance in chronic myeloid leukaemia cells. Cell proliferation was determined by trypan blue dye exclusion test. Western blot analysis was performed to test the expression of key proteins. EGCG showed anti-proliferative effects in TCCY cells (IC50 = 23 μM), TCCY/T315I cells (IC50 = 19 μM) and wild type and mutant BCR-ABL-transfected Ba/F3 cells (IC50 from 30 to 33 μM). Moreover, treatment with EGCG (4 hours) resulted in decrease of BCR-ABL expression. Finally, EGCG treatment inhibited the phosphorylation of AKT and MAPK and induced apoptosis in these cells.