Open AccessRedesigning Primer of ITS2 (Internal Transcribed Spacer 2) for Specific Molecular Characterization of Malaria Vectors Anopheles Species
Author(s) -
Kartika Senjarini,
Muhammad Abdullah,
Nuril Azizah,
Miatin Alvin Septianasari,
Ahmad Tosin,
Rike Oktarianti,
Syubbanul Wathon,
Tri Yudani Mardining Raras
Publication year - 2021
Publication title -
medicinski arhiv
Language(s) - English
Resource type - Journals
eISSN - 1986-5961
pISSN - 0350-199X
DOI - 10.5455/medarh.2021.75.418-423
Subject(s) - biology , anopheles , primer (cosmetics) , internal transcribed spacer , anopheles stephensi , vector (molecular biology) , genetics , genome , anopheles albimanus , malaria , zoology , botany , larva , gene , ribosomal rna , aedes aegypti , chemistry , organic chemistry , immunology , recombinant dna
Anopheles mosquitoes are vectors of malaria, which is a serious health issue in Indonesia. Thus, vector control is an important approach taken to overcome this disease. The first and most important step in vector control is vector identification. As some Anopheles species share similar morphological features, molecular identification helps make the process more accurate by using specific DNA sequences as molecular markers such as Internal Transcribed Spacer 2 (ITS2). Many of the available ITS2 primers are universally designed for insects and, as such, are typically less specific for identifying certain genera, such as Anopheles sp. Therefore, redesigning a specific ITS2 primer is needed for specific Anopheles identification.