Open AccessCharacterization of a New Thermoalkalophilic Xylanase-Producing Bacterial Strain Isolated from Cimanggu Hot Spring,West Java, Indonesia
Author(s) -
Maria Ulfah,
Is Helianti,
Budiasih Wahyuntari,
Niknik Nurhayati
Publication year - 2011
Publication title -
microbiology indonesia
Language(s) - English
Resource type - Journals
eISSN - 2087-8575
pISSN - 1978-3477
DOI - 10.5454/mi.5.3.7
Subject(s) - hot spring , bacteria , 16s ribosomal rna , xylanase , strain (injury) , amylase , thermophile , microbiology and biotechnology , biology , protease , enzyme , bacillus (shape) , food science , biochemistry , anatomy , paleontology , genetics
Alkalophilic bacteria and their enzymes are important for industrial applications. Therefore, finding out new strains of alkalophilic bacteria from Indonesian microbial diversity is still required. In this study, a thermoalkalophilic bacterium was isolated from sediment of local hot spring, Cimanggu,West Java. The temperature of the spot was 60 °C and the pH was 8. The bacterium could live at pH 11 and temperature 55 °C, and produced xylanase that have optimal activity at alkaline pH 9 and high temperature 70 °C. Based on the analyses of 16S rRNA sequence similarity and biochemical characteristics, this strain was clustered into the same group of Bacillus halodurans species with 99% identity to Bacillus halodurans C-125. The isolate also showed other enzyme activities such as amylase, protease, and gelatinase, promising its potential use as an industrial enzymes producer