Characterization of a New Thermoalkalophilic Xylanase-Producing Bacterial Strain Isolated from Cimanggu Hot Spring,West Java, Indonesia | Zendy

Maria Ulfah | Zendy; Is Helianti | Zendy; Budiasih Wahyuntari | Zendy; Niknik Nurhayati | Zendy

Open Access

Characterization of a New Thermoalkalophilic Xylanase-Producing Bacterial Strain Isolated from Cimanggu Hot Spring,West Java, Indonesia

Author(s) -

Maria Ulfah,

Is Helianti,

Budiasih Wahyuntari,

Niknik Nurhayati

Publication year - 2011

Publication title -

microbiology indonesia

Language(s) - English

Resource type - Journals

eISSN - 2087-8575

pISSN - 1978-3477

DOI - 10.5454/mi.5.3.7

Subject(s) - hot spring , bacteria , 16s ribosomal rna , xylanase , strain (injury) , amylase , thermophile , microbiology and biotechnology , biology , protease , enzyme , bacillus (shape) , food science , biochemistry , anatomy , paleontology , genetics

Alkalophilic bacteria and their enzymes are important for industrial applications. Therefore, finding out new strains of alkalophilic bacteria from Indonesian microbial diversity is still required. In this study, a thermoalkalophilic bacterium was isolated from sediment of local hot spring, Cimanggu,West Java. The temperature of the spot was 60 °C and the pH was 8. The bacterium could live at pH 11 and temperature 55 °C, and produced xylanase that have optimal activity at alkaline pH 9 and high temperature 70 °C. Based on the analyses of 16S rRNA sequence similarity and biochemical characteristics, this strain was clustered into the same group of Bacillus halodurans species with 99% identity to Bacillus halodurans C-125. The isolate also showed other enzyme activities such as amylase, protease, and gelatinase, promising its potential use as an industrial enzymes producer

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