Open AccessPhytochemical Analysis and Antibacterial Activity of the Kenyan Wild Orchids
Author(s) -
Polycarp Chagona,
Nancy Kwamboka,
Humphrey Gaya,
Huxley Mae Makonde,
Aggrey Adem,
Kenneth Osano,
Fanuel Kawaka
Publication year - 2021
Publication title -
micro environer
Language(s) - English
Resource type - Journals
ISSN - 2783-3526
DOI - 10.54458/mev.v1i02.6677
Subject(s) - antibacterial activity , phytochemical , bacillus subtilis , agar diffusion test , terpenoid , biology , pseudomonas aeruginosa , traditional medicine , microbiology and biotechnology , glycoside , staphylococcus aureus , bacteria , chemistry , botany , medicine , genetics
The current study evaluated the antibacterial activity of dichloromethaneand methanol (DCM-MeOH) extracts of four Kenyan orchid species againstthree selected bacterial strains. Extracts of E1 (Ansellia Africana), E2(Trydactylescottelli), E3 (Polystachyabella) and E4 (Liparis bowkeri) werescreened for antibacterial activity against staphylococcus aureus, Bacillussubtilis and Pseudomonas aeruginosa using agar disc diffusion. Ampicillinwas included as a positive control. Qualitative analysis revealed thepresence of flavonoids, saponins, alkaloids, tannins, terpenoids, steroidsand glycosides. Trydactyle scottelli and Polystachyabella extracts revealed amore substantial presence of tannins and steroids, respectively, comparedto others. All extracts showed varying levels of antibacterial activityagainst the test bacteria. However, Polystachyabella and Liparis bowkeriagainst Bacillus subtilis and Ansellia Africana against Pseudomonasaeruginosa exhibited higher activities similar to that of Ampicillin. Thestudy further showed that the DCM-MeOH extracts of the four orchidscontain potential compounds that should be screened for conventionalmanagement of bacterial infections.