Open AccessComparison of GeneXpert assay and Fluorescent Microscopy for the Diagnosis of Pulmonary Tuberculosis in Narowal region
Author(s) -
Asma Amjad,
Farheen Ansari,
Azhar Iqbal,
Qurat Ul Ain,
Ali Ahsan,
Sadia Aziz,
Zeeshan Yusuf
Publication year - 2021
Publication title -
markhor
Language(s) - English
Resource type - Journals
eISSN - 2790-4385
pISSN - 2790-4377
DOI - 10.54393/mjz.v2i2.21
Subject(s) - genexpert mtb/rif , tuberculosis , mycobacterium tuberculosis , fluorescence , medicine , pulmonary tuberculosis , fluorescence microscope , tuberculosis diagnosis , pathology , optics , physics
GeneXpert is attributed as one of the latest technical means for diagnosing Tuberculosis in very short period of time. Objectives: To evaluate the efficiency of GeneXpert and fluorescent microscopy in the detection of pulmonary tuberculosis (TB); To compare the sensitivity and specificity of GeneXpert and fluorescent microscope in the diagnosis of Mycobacterium tuberculosis Methods: In the present investigation, the diagnostic performance of GeneXpert MTB / RIF for tuberculosis was compared with the performance of light-emitting diode fluorescence microscope in TB samples from Narowal, Pakistan. For this purpose, a total of 299 TB positive specimens were obtained. Among these 54% (n = 160) were categorized to be obtained from male and 46% (n = 139) from female population. Data collected was distributed in 4 age groups; 0-20, 21-40, 41-60, and 61-80, in which the percentage and number of samples were found as 2% (n = 6), 60% (n = 179), 27% (n = 80) and 11% (n = 34), respectively. Results: The parameters including sensitivity and specificity calculated for GeneXpert were 73% and 100%, correspondingly, while the sensitivity and specificity calculated for LED-FM microscope were 43% and 100%, respectively. Conclusions: This indicates that the GeneXpert is more sensitive in detecting MTB in comparison to LED-FM technique. The GeneXpert assay was also found to detect small number of bacillus from samples in comparison to LED-FM method