Open AccessRisk factors associated with the occurrence of multiresistant Staphylococcus spp. isolated from bovine subclinical mastitis in northern Brazil
Author(s) -
Lilian Bernardina Ferreira,
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Larissa de Freitas Santiago Israel,
Renata Fernandes Rabello,
Guilherme Nunes de Souza,
Rui Carlos Peruquetti,
Luciana dos Santos Medeiros,
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AUTHOR_ID,
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AUTHOR_ID,
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Publication year - 2022
Publication title -
semina. ciências agrárias
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.268
H-Index - 25
eISSN - 1679-0359
pISSN - 1676-546X
DOI - 10.5433/1679-0359.2022v43n2p901
Subject(s) - mastitis , biology , staphylococcus , veterinary medicine , subclinical infection , antibiotic resistance , multiple drug resistance , california mastitis test , microbiology and biotechnology , antimicrobial , staphylococcus aureus , epidemiology , drug resistance , medicine , antibiotics , bacteria , virology , lactation , pregnancy , genetics , ice calving
Staphylococcus bacteria are often associated with subclinical bovine mastitis. This study aimed to identify multiresistant Staphylococcus spp. associated with subclinical mastitis and the associated risk factors. Twenty-three dairy farms with a history of decrease in milk production, located in the lower Acre region, Brazil, were selected. An epidemiological questionnaire was provided in all farms. All animals were examined using the California Mastitis Test (CMT) and their milk samples were collected for bacterial culture. After isolation and identification, the disk diffusion antimicrobial susceptibility test was performed against nine classes of antimicrobials. Of the 339 cows examined using the CMT, 108 had mastitis. A total of 229 milk samples were collected from individual teats. MALDI-TOF MS found isolates belonging to eight species of Staphylococcus, in 101 of these samples. S. chromogenes (58.4%) demonstrated strongest resistance to the nine classes of antimicrobial active principles. Nineteen isolates with multidrug resistance phenotypic profile were identified. This phenotypic expression indicates wide circulation of resistant genes in this species. The presence of multidrug resistance in Staphylococcus spp. in this study was correlated with lack of water for cleaning the corral, which is a preventive factor, minimizing the transmission and persistence of pathogens in the farms.