Open AccessDocosahexaenoic acid associated vitamin E in the diluent for cryopreservation of goat semen
Author(s) -
Caline Santana da França,
Poliana Almeida Bezerra,
Claudinéia Silva Mendes,
Laiara Fernandes Rocha,
Ana Lúcia Almeida Santana,
Rosiléia Silva Souza,
Rodrigo Freitas Bittencourt,
William Morais Machado,
Paola Pereira das Neves Snoeck,
Larissa Pires Barbosa
Publication year - 2021
Publication title -
semina. ciências agrárias
Language(s) - English
Resource type - Journals
eISSN - 1679-0359
pISSN - 1676-546X
DOI - 10.5433/1679-0359.2021v42n1p255
Subject(s) - semen , cryopreservation , andrology , sperm , semen analysis , semen quality , diluent , extender , docosahexaenoic acid , biology , sperm motility , semen collection , semen extender , chemistry , artificial insemination , biochemistry , fatty acid , medicine , polyunsaturated fatty acid , infertility , embryo , pregnancy , genetics , organic chemistry , nuclear chemistry , polyurethane , microbiology and biotechnology
The objective of this study was to evaluate the effect of and determine the optimum level of inclusion of docosahexaenoic acid (DHA) in the diluent for goat semen cryopreservation. Five Boer males underwent semen collection, totaling 10 viable collections per animal. After evaluation, the ejaculates were pooled and fractionated in Tris-yolk medium with the addition of 0; 30; 45; or 60ng mL-1 of DHA and 0.4 mmol of alpha-tocopherol (Vitamin E). The semen was cryopreserved in a freezing machine (TK 3000TM) and placed in a cryogenic cylinder for subsequent analysis. Data were evaluated by regression analysis at 5% significance. There were no differences (P > 0.05) in sperm kinetic parameters evaluated by computer assisted sperm analysis: total motility (79.17 ± 17.31%), progressive motility (14.04 ± 5.73%), curvilinear speed (58.82 ± 6.35µm/s), progressive linear speed (22.49 ± 3.63µm/s), mean path speed (35.17 ± 4.52µm/s), linearity (38.69 ± 5.79%), rectilinearity (63.99 ± 6.64%), and oscillation index (59.68 ± 2.99%). There were no differences (P > 0.05) found from the membrane functional integrity test for reactive spermatozoa (69.66 ± 9.76%), plasma and acrosomal membrane integrity of intact spermatozoa (29.86 ± 7.57%), mitochondrial potential of Class I cryopreserved goat semen (72.75 ± 9.81%), and chromatin compaction of intact chromatin (96.87 ± 4.37%). Thus, the inclusion of up to 60ng mL-1 of DHA did not promote any improvement in the seminal quality parameters of post-thawed goat semen.