Open AccessEffect of the addition of sodium caseinate on the viability of cryopreserved buffalo semen
Author(s) -
Fernando Evaristo da Silva,
Jaqueline Candido de Carvalho,
Camila de Paula Freitas Dell’Aqua,
Frederico Ozanam Papa,
M. Henry,
Eunice Oba,
João Carlos Pinheiro Ferreira
Publication year - 2020
Publication title -
semina. ciências agrárias
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.268
H-Index - 25
eISSN - 1679-0359
pISSN - 1676-546X
DOI - 10.5433/1679-0359.2020v41n5supl1p2209
Subject(s) - cryopreservation , semen , diluent , andrology , sperm , insemination , yolk , chemistry , semen cryopreservation , sodium , artificial insemination , skimmed milk , capacitation , cryoprotectant , biology , sperm motility , food science , embryo , pregnancy , medicine , microbiology and biotechnology , genetics , organic chemistry , nuclear chemistry
The use of cooled semen in artificial insemination operations results in higher pregnancy rates than the use of frozen semen. This result seems to be related to the more severe damage triggered by the freezing process than that observed during refrigeration. Due to its ability to bind to sperm-binding proteins and calcium ions, sodium caseinate has been studied as a substance capable of preventing early sperm capacitation, a significant cause of the decreased pregnancy rate resulting from the use of frozen semen. The first objective of this study was to evaluate whether a commercial egg yolk diluent developed for frozen bovine semen could be used for buffalo semen cryopreservation; the second objective was to investigate the effect of this diluent in combination with sodium caseinate during the procedures of buffalo sperm cryopreservation using flow cytometry and computer-assisted sperm analysis. In the first part of the study, comparing the results of spermatic kinetics and plasma and acrosomal membrane integrity, it was observed that the freezing process resulted in more cell damage than the cooling process. In the second part of the study, no effects of the addition of sodium caseinate to the egg yolk diluent were observed. From the results of the present study, it was possible to conclude that the egg yolk-based diluent was suitable for buffalo semen cryopreservation and that the addition of sodium caseinate did not decrease the harmful effects related to seminal cryopreservation.