Open AccessPrimer design for complete sequencing of the Avastrovirus ORF2 gene
Author(s) -
Luis Luna Espinoza,
Laila Andreia Rodrigues Beserra,
Rodrigo Martins Soares,
Fábio Gregori
Publication year - 2018
Publication title -
semina. ciências agrárias
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.268
H-Index - 25
eISSN - 1679-0359
pISSN - 1676-546X
DOI - 10.5433/1679-0359.2018v39n1p411
Subject(s) - biology , sanger sequencing , virology , primer (cosmetics) , dna sequencing , gene , genetics , genotype , computational biology , chemistry , organic chemistry
Avastrovirus infection is associated with enteric disease, nephritis, and hepatitis in birds. In this study, we present a protocol for the complete sequencing of the ORF2 gene of avian nephritis virus (ANV), chicken astrovirus (CAstV), and turkey astrovirus type 1 (TAstV-1) using a conventional Sanger technique. Previously and newly designed primer pairs targeting both the conserved flanking and internal regions of the ORF2 gene of these three viruses were used. The information derived from the astroviral sequences obtained in this study is fundamental for characterizing this virus and providing data regarding several aspects of disease epidemiology and prevention.