Open AccessAnalysis of the acid and neutral fractions present in Carramboa tachirensis (Aristeg.) Cuatrec. (Asteraceae) by Gas Chromatography-Mass Spectrometry
Author(s) -
Ysbelia M Obregón,
Luis Rojas,
Rosa Aparicio,
Alfredo Usubillaga
Publication year - 2021
Publication title -
revista de la facultad de farmacia
Language(s) - English
Resource type - Journals
eISSN - 2739-0373
pISSN - 0041-8307
DOI - 10.54305/rffucv.2021.84.1-2.11
Subject(s) - asteraceae , mass spectrometry , chromatography , hexane , gas chromatography–mass spectrometry , gas chromatography , chemistry , ether , diethyl ether , organic chemistry , biology , botany
The genus Carramboa (family Asteraceae) comprises seven species, among which is the Carramboa tachirensis (Aristeg.) (Cuatrec.), species endemic to the Andean paramos. In this paper we prepared an extract of n-hexane: diethyl ether (3:1) from dried leaves of the plant then followed a march acid-base, yielding two fractions: an acid, which was methylated, and one neutral. Both were analyzed by Gas Chromatography-Mass Spectrometry. The identification was made by comparison with authentic standards and with the use of databases Wiley (6th Edition), Nist 05, and Adams. Compounds identified were: ent-kaur-16-en-19-oic acid (11.16 %), ent-kauran-19-oic acid (9.18 %) and ent-15α-hydroxy-16-en-kaur-19-oic acid (17.03 %) of the acid fraction and ent-kaur-16-en-19-ol (2.63 %), epi-ruilopeziol (5.19 %) and 16