Open AccessIdentification of death receptors DR4 and DR5 in HTB-12 astrocytoma cell lines and determination of TRAIL sensitivity
Author(s) -
Elenia Riddick,
S Evans,
Jeffrey M. Rousch,
Ephraim T. Gwebu,
Hirendra Nath Banerjee
Publication year - 2013
Publication title -
journal of solid tumors
Language(s) - English
Resource type - Journals
eISSN - 1925-4075
pISSN - 1925-4067
DOI - 10.5430/jst.v3n6p20
Subject(s) - trypan blue , receptor , cytotoxicity , cytotoxic t cell , apoptosis , astrocytoma , programmed cell death , cell culture , cancer research , tumor necrosis factor alpha , necrosis , microbiology and biotechnology , medicine , chemistry , immunology , biology , glioma , pathology , biochemistry , in vitro , genetics
Astrocytomas are tumors which arise from astrocytes, cells that form the blood-brain barrier. There are very few drugs that successfully treat brain tumors. In this study, the cytotoxic effects on the HTB-12 astrocytoma cell line by tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) were studied. The presence of the TRAIL receptors, Death receptor 4 (DR4) and Death receptor 5 (DR5), were detected in HTB-12 cells by Enzyme-Linked Immunosorbent Assay (ELISA). Cytotoxicity assay by Trypan Blue Exclusion Method showed effective cell killing by TRAIL treatment. Thus, the presence of death receptors and TRAIL efficacy raises the therapeutic potential for this type of brain tumor.