Open AccessThe application of sequence specific primer and RT-PCR to LRRK2 gene polymorphism typing
Author(s) -
G Biao,
Tian Gai-sheng,
L Qinxue,
L Fengrui
Publication year - 2019
Publication title -
discussion of clinical cases
Language(s) - English
Resource type - Journals
eISSN - 2375-8449
pISSN - 2375-8473
DOI - 10.5430/dcc.v6n2p17
Subject(s) - genotyping , biology , genotype , primer (cosmetics) , polymerase chain reaction , genetics , typing , gene , microbiology and biotechnology , chemistry , organic chemistry
Objective: To establish a new detecting method for disease susceptibility loci R1628P and G2385R of Parkinson’s disease (PD) related gene LRRK2.Methods: Sequence specific primers were designed to make a genotyping of DNA markers with known genotypes by use of quantitative fluorescence real-time PCR (RT-PCR). 100 cases of PD samples with unknown genotypes were tested, and verified by use of polymerase chain reaction linked restriction fragment length polymorphism (PCR-RLFP).Results: The genotyping results of DNA markers proved to be correct, and 100 cases of samples to be tested had a completely consistent genotyping result with PCR-RLFP genotyping result.Conclusions: Sequence specific primer and quantitative fluorescence RT-PCR can successfully make a genotyping for disease susceptibility loci R1628P and G2385R of LRRK2.