Open AccessEffect of Asiatic mangrove plant (Rhizophora mucronata) extract on the growth and virulence of Vibrio harveyi causing bioluminescence disease in Penaeus monodon larviculture
Author(s) -
S. Kannappan,
K. Sivakumar,
K. P. Jithendran,
Sivamani Balasubramaniam,
P. Ezhil Praveena
Publication year - 2021
Publication title -
spanish journal of agricultural research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.337
H-Index - 36
eISSN - 2171-9292
pISSN - 1695-971X
DOI - 10.5424/sjar/2021193-17044
Subject(s) - vibrio harveyi , rhizophora mucronata , penaeus monodon , biology , veterinary medicine , vibrio , shrimp , mangrove , botany , microbiology and biotechnology , bacteria , ecology , medicine , genetics
Aim of the study: Vibrio harveyi bacteria are affecting shrimps during grow-out practices. The application of chemicals to control V. harveyi has resulted in antibiotic‐resistance among bacteria. An extract of the leaves of Rhizophora mucronata was tested to control the growth and virulences of V. harveyi. Area of study: This study was conducted in the Crustacean Culture Division of ICAR-CIBA, Chennai city, Tamil Nadu State, India. Material and methods: R. mucronata plants were collected from the Pitchavaram area, and the contents extracted. The resultant extract was prepared and tested against the growth of V. harveyi and its virulence factors. The various functional compounds of R. mucronata were screened and volatile compounds were analyzed. Main results: When R. mucronata extract was treated against V. harveyi (350 µg/mL) an inhibitory zone of 14 ± 0.1 mm was observed. At 300 µg/mL, the extract was found to be active in decreasing the luciferase to a maximum of 76 counts per second in 30 days and a similar level of bioluminescence was reduced in 15 days. During, shrimp larviculture a reduction in the cumulative percent of mortality 15.70% (p<0.033) was observed when treated with the extract of R. mucronata. Research highlights: When extract (200 μg/mL) of R. mucronata was tested against V. harveyi during Penaeus monodon larviculture, the V. harveyi counts decreased (p<0.049). Volatile compounds viz, tetramethyl-6,7,8,8a-tetrahydro-5H-naphthalene-1-one (38.63%), squalene (31.19%), α-amyrin, (7.07%) and β-amyrin (8.75%) were detected. It would be desirable to use crude extracts of R. mucronata during shrimp culture to control V. harveyi.