Open AccessPinus canariensis plant regeneration through somatic embryogenesis
Author(s) -
Ander Castander-Olarieta,
Paloma Moncaleán,
Itziar Aurora Montalbán
Publication year - 2020
Publication title -
forest systems
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.455
H-Index - 25
eISSN - 2171-9845
pISSN - 2171-5068
DOI - 10.5424/fs/2020291-16136
Subject(s) - somatic embryogenesis , biology , micropropagation , botany , subculture (biology) , acclimatization , germination , somatic cell , embryo , tissue culture , embryogenesis , microbiology and biotechnology , in vitro , biochemistry , gene
Aim of the study: To develop an efficient method to regenerate plants through somatic embryogenesis of an ecologically relevant tree species such as Pinus canariensis.Area of study: The study was conducted in the research laboratories of Neiker-Tecnalia (Arkaute, Spain).Material and methods: Green cones of Pinus canariensis from two collection dates were processed and the resulting immature zygotic embryos were cultured on three basal media. The initiated embryogenic tissues were proliferated testing two subculture frequencies, and the obtained embryogenic cell lines were subjected to maturation. Germination of the produced somatic embryos was conducted and acclimatization was carried out in a greenhouse under controlled conditions.Main results: Actively proliferating embryogenic cell lines were obtained and well-formed somatic embryos that successfully germinated were acclimatized in the greenhouse showing a proper growth.Research highlights: This is the first report on Pinus canariensis somatic embryogenesis, opening the way for a powerful biotechnological tool for both research purposes and massive vegetative propagation of this species.Keywords: acclimatization; Canary Island pine; micropropagation; embryogenic tissue; somatic embryo.Abbreviations used: embryogenic tissue (ET); established cell line (ECL); somatic embryogenesis (SE); somatic embryos (Se’s).