Open AccessRapid Detection Method for Fusaric Acid-producing Species of Fusarium by PCR
Author(s) -
Theresa Lee,
Sosoo Kim,
Mark Busman,
Robert H. Proctor,
Hyeonhui Ham,
Soohyung Lee,
Sung Kee Hong,
Jae-Gee Ryu
Publication year - 2015
Publication title -
singmulbyeong yeon-gu
Language(s) - English
Resource type - Journals
eISSN - 2233-9191
pISSN - 1598-2262
DOI - 10.5423/rpd.2015.21.4.326
Subject(s) - fusaric acid , fusarium proliferatum , fusarium , beauvericin , biology , multiplex polymerase chain reaction , fusarium oxysporum , multiplex , microbiology and biotechnology , polymerase chain reaction , botany , gene , genetics
Fusaric acid is a mycotoxin produced by species of the fungus Fusarium and can act synergistically with other Fusarium toxins. In order to develop a specific detection method for fusaric acid-producing fungus, PCR primers were designed to amplify FUB10, a transcription factor gene in fusaric acid biosynthetic gene cluster. When PCR with Fub10-f and Fub10-r was performed, a single band (~550 bp) was amplified from F. oxysporum, F. proliferatum, F. verticillioides, F. anthophilum, F. bulbicola, F. circinatum, F. fujikuroi, F. redolens, F. sacchari, F. subglutinans, and F. thapsinum, all of which were known for fusaric acid production. Whereas the FUB10 specific band was not amplified from Fusarium species known to be trichothecene producer. Because production of fusaric acid can co-occur in species that also produce fumonisin mycotoxins, we developed a multiplex PCR assay using the FUB10 primers as well as primers for the fumonisin biosynthetic gene FUM1. The assay yielded amplicons from fumonisin producers such as F. proliferatum and F. verticillioides, allowing for the simultaneous detection of species with the genetic potential to produce both types of mycotoxins.\u