Open AccessCharacterization of Burkholderia glumae Putative Virulence Factor 11 (PVF11) via Yeast Two-Hybrid Interaction and Phenotypic Analysis
Author(s) -
Juyun Kim,
Namgyu Kim,
Mohamed Mannaa,
HyunHee Lee,
JongSeong Jeon,
Young-Su Seo
Publication year - 2019
Publication title -
plant pathology journal/the plant pathology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.542
H-Index - 29
eISSN - 2093-9280
pISSN - 1598-2254
DOI - 10.5423/ppj.nt.02.2019.0026
Subject(s) - virulence factor , virulence , phenotype , genetics , biology , yeast , burkholderia , microbiology and biotechnology , gene , bacteria
In this study, PVF11 was selected among 20 candidate pathogenesis-related genes in Burkholderia glumae based on its effect on virulence to rice. PVF11 was found to interact with several plant defense-related WRKY proteins as evidenced through yeast-two hybrid analysis (Y2H). Moreover, PVF11 showed interactions with abiotic and biotic stress response-related rice proteins, as shown by genome-wide Y2H screening employing PVF11 and a cDNA library from B. glumae- infected rice. To confirm the effect of PVF11 on B. glumae virulence, in planta assays were conducted at different stages of rice growth. As a result, a PVF11-defective mutant showed reduced virulence in rice seedlings and stems but not in rice panicles, indicating that PVF11 involvement in B. glumae virulence in rice is stage-dependent.