Open AccessUsing in silico RFLP method for the study of MC1R gene alleles in the species Sus Scrofa
Author(s) -
Victor Sîtnic
Publication year - 2021
Language(s) - English
Resource type - Conference proceedings
DOI - 10.53937/icz10.2021.61
Subject(s) - genotyping , introgression , restriction fragment length polymorphism , biology , restriction enzyme , genetics , backcrossing , allele , hybrid , gene , polymorphism (computer science) , evolutionary biology , genotype , botany
. In the present study we aimed to use a bioinformatics algorithm that predict RFLP fragmentation of swine MC1R alleles simulating the sequence digestion with over 700 restriction enzymes. The results show several restriction enzymes that have the potential to be used for genotyping of Sus scrofa individuals and for differentiation between hybrids and pure line wild boars (WB)/domestic pigs (DP). Genotyping studies of wild boars and domestic pigs in Moldova populations would allow the determination of introgression/backcrossing degree and would contribute to the elaboration of adequate conservation measures