Open AccessEffect ropivacaine infiltration in incisional wound on caspase-3 expression and apoptosis squamous cells wound healing
Author(s) -
Abdurrahman Abdurrahman,
Prananda Surya Airlangga,
Imam Susilo
Publication year - 2022
Publication title -
international journal of health sciences (ijhs) (en línea)
Language(s) - English
Resource type - Journals
eISSN - 2550-6978
pISSN - 2550-696X
DOI - 10.53730/ijhs.v6ns4.6148
Subject(s) - infiltration (hvac) , wound healing , apoptosis , ropivacaine , medicine , caspase 3 , cellular infiltration , inflammation , surgery , anesthesia , andrology , pathology , chemistry , programmed cell death , biochemistry , physics , thermodynamics
Wound healing and tissue regeneration consist of molecular and cellular process that occur right after wound formation. The intrinsic pathway of apoptosis, apoptosome produced to initiate the formation of Caspase 3 important role in apoptosis. One of the methods that accelerates wound healing process by eliminating pain by infiltration of local anesthesia. This study aims to determine the effect of ropivacaine infiltration as local anaesthesia in the expression of Caspase 3 and apoptosis. This study is an experimental study with randomized post-test only control group design. The samples of this study were 24 wistar rats. The rats divided into four groups then incised, either followed by 1 ml ropivacaine 0.2% infiltration or left as is. Excisional biopsy procedures were done on third and seventh day. Then analyzed by SPSS. Expression of Caspase 3 on squamous cell epithel of control and intervention group was found statistically different on day 3 (p=0.024) and day 7 (p=0.041). Number of apoptosis on squamous cell epithel of control and intervention was found statistically different on day 7 (p=0.031) but not on day 7 (p=0.414). Ropivacaine infiltration on incision wound decreased the expression of Caspase 3 on day 3 and 7; and apoptosis on day 7.