Open AccessMolecular detection and Phylogenetic study role of ORF1ab in SARS-COV2 on patients in Hilla city
Author(s) -
Noor A. Jawad,
Zaytoon A. Al-Khafaji,
Bushra J. Al-Tamimi
Publication year - 2022
Publication title -
international journal of health sciences (ijhs) (en línea)
Language(s) - English
Resource type - Journals
eISSN - 2550-6978
pISSN - 2550-696X
DOI - 10.53730/ijhs.v6ns3.6693
Subject(s) - orfs , phylogenetic tree , genome , biology , gene , coding region , genetics , virus , open reading frame , virology , pandemic , covid-19 , medicine , peptide sequence , disease , infectious disease (medical specialty) , pathology
The Covid-19 pandemic, which is happening all over the world, needs serious attention. Covid-19 initiated by an RNA type virus can cause more severe problems later on due to its ability to mutate. CoV genomes code for a ORF1a / ORF1ab polyprotein and four structural proteins widely studied as major drug targets. The genomes also contain a variable number of open reading frames (ORFs) coding for accessory proteins that are not essential for virus replication, but appear to have a role in pathogenesis. This study was conducted on 50 nasopharyngeal swaps from Morjan teaching Hospital using RT-PCR and PCR for ORF1ab gene amplification after that, five samples were subjected the phylogenetic analysis. The results are confirmed by the ORF1ab analysis shows that the ORF1ab vary and mutated and have a number of mutation tht could affect it similarity between countries in aimed of detection our local isolate were had a 100 identities with United State concluding that the extreme effect of virus transmission and it is mobile genetic holding.