Open AccessMolecular detection and genotyping of Taenia hydatigena between intermediate (sheep and goats) and final hosts (stray dogs) in Baghdad city
Author(s) -
Gharib Dohi Yaseen Al-Sudani,
Amer Murhum Al-Amery
Publication year - 2022
Publication title -
international journal of health sciences (ijhs) (en línea)
Language(s) - English
Resource type - Journals
eISSN - 2550-6978
pISSN - 2550-696X
DOI - 10.53730/ijhs.v6ns3.5402
Subject(s) - taenia hydatigena , biology , genotyping , veterinary medicine , parasite hosting , dna extraction , agarose gel electrophoresis , zoology , polymerase chain reaction , gene , genotype , helminths , genetics , cestode infections , medicine , world wide web , computer science
Taenia hydatigena cestode parasite found in the intestine of dogs and larval stage Cysticercus tenuicollis located in visceral organs of intermediate hosts (sheep and goats). In this study, investigate the genetic variation among the recovered isolates from sheep, goats, and stray dogs in Baghdad city, based on the mitochondrial cox1 gene. After DNA extraction, the PCR that was used indicated the specificity at the band (450bp) on the agarose gel. Sequencing and phylogenetic results were divided into three groups depending on the closed relationship between them and to NCBI-Blast T. hydatigena.