Open AccessIsolation and characterization of MDR- Klebsiella pneumonia phage from Euphrates River in Al-Anbar city
Author(s) -
Saad. T. Mutlk
Publication year - 2022
Publication title -
international journal of health sciences (ijhs) (en línea)
Language(s) - English
Resource type - Journals
eISSN - 2550-6978
pISSN - 2550-696X
DOI - 10.53730/ijhs.v6ns2.6208
Subject(s) - klebsiella pneumonia , klebsiella , isolation (microbiology) , macconkey agar , antibiotics , microbiology and biotechnology , sewage , antibiotic sensitivity , biology , veterinary medicine , geography , agar plate , bacteria , medicine , staphylococcus aureus , environmental science , escherichia coli , environmental engineering , biochemistry , gene , genetics
In Iraq, there is no active action for Al-Ramadi teaching hospital for maternity and children waste which is straightforwardly discharge in to Euphrates River in Al-Anbar city. As a result, the numbers of multi-drugs resistant bacteria were raised specially in Enterobacteriaceae family (Klebsiella pneumonia). Nevertheless, modern studies are using phages isolated from similar ecosystem as a biologic alternate for antimicrobic remedy. The present work has an aim to isolate Klebsiella pneumonia and categorise its antibiotic resistance elements from Euphrates River in Al-Anbar city, Iraq. As well as isolation of phages from the similar collected river water specimens then examining their repressive consequence on MDR- Klebsiella pneumonia isolates. Water specimens were taken from 5 sites in to Euphrates River near to the residues of sewage estuaries that run straight to the main river for many regions of Ramadi city. These specimens were inoculated on MacConkey agar plates for morphologic appearance, and biochemical characters by API- 20E test and confirmed by VITEK 2. Disc diffusion technique was performed to identify antibiotic sensitivity.