Open AccessExosomal gene fold expression estimation of Leishmania major by real time qPCR in vivo and in vitro
Author(s) -
Manahel Hadi Mjefi,
Muthaneemah Karam,
Zainab Abdul-Gabbar,
Ali Shakir K. Al-Suraifi
Publication year - 2022
Publication title -
international journal of health sciences (ijhs) (en línea)
Language(s) - English
Resource type - Journals
eISSN - 2550-6978
pISSN - 2550-696X
DOI - 10.53730/ijhs.v6ns2.5695
Subject(s) - gene expression , gene , biology , in vivo , in vitro , exosome , microbiology and biotechnology , cell culture , genetics , microvesicles , microrna
The aim of this research is to estimate the virulence factor L. major exosome by real time gene expression. The higher gene expression in footpad tissue was (29.34), the higher gene expression in day seven culture was (20.37), higher gene expression in day twelve culture was (12.25), highest gene expression in culture line control (8.33). The current work revealed that exosome gene expression was elevated in footpad in comparison to cultures media. The elevated levels were in passage three and lowest levels in passage one, in control culture line the level of gene expression was declined with progress of time.