Open AccessStudies on Cytotoxic and Genotoxic potential of Ethanolic extract of Lawsonia inermis leaves
Author(s) -
Mohammad Munawar T,
Chinta Koteswara Rao,
Andanamala Vijay Rajesh,
Udit Narayan Sharma,
Istuti Gupta,
Dhan Prakash
Publication year - 2022
Publication title -
international journal of health sciences (ijhs) (en línea)
Language(s) - English
Resource type - Journals
eISSN - 2550-6978
pISSN - 2550-696X
DOI - 10.53730/ijhs.v6ns1.5842
Subject(s) - lawsonia inermis , lythraceae , genotoxicity , traditional medicine , sister chromatid exchange , mitotic index , biology , chemistry , toxicology , botany , biochemistry , toxicity , in vitro , medicine , mitosis , genetics , organic chemistry
Lawsonia inermis is widely known as henna and belongs to Lythraceae family. It contains various phytochemica constituents in every part of plant and shows various pharmacological properties. Secondary metabolites are acts as antioxidants and these are very high in this plant species. In this research studies, ethanolic extract of Lawsonia inermis was performed in the blood sample culture for cytotoxicity and genotoxicity effects. The both of the effects of plant extracts were evaluated through measurement of mitotic index (MI), sister chromatid exchanges (SCE) and cell proliferation kinetics (CPK). The blood sample culture was treated with control and various concentrations of extracts (500μg/ml and 1000μg/ml). The mean of MI, SCE and CPK for control were found to be 4.51, 5.19 and 0.52 respectively where as for 500μg/ml of Lawsonia inermis extracts are 4.33, 4.87 and 0.50 respectively and for 1000 μg/ml extracts are 4.18, 4.68 and 0.48 respectively. From the results, we can conclude that the MI and frequency of SCES of ethanolic plant extracts are remarkably similar to that of control which indicates that the Lawsonia inermis plant extracts have no substantial cytotoxicity and genotoxicity effects in cultured blood lymphocytes.