Open AccessRetinol rescues immune cells from inflammation in bacterial infection
Author(s) -
Aare Nichita,
Pawan Kumar Anoor,
Sandeepta Burgula
Publication year - 2022
Publication title -
international journal of health sciences (ijhs) (en línea)
Language(s) - English
Resource type - Journals
eISSN - 2550-6978
pISSN - 2550-696X
DOI - 10.53730/ijhs.v6ns1.5328
Subject(s) - inflammation , immune system , cytokine , tlr4 , immunology , retinol , peripheral blood mononuclear cell , medicine , interleukin 10 , vitamin , tumor necrosis factor alpha , biology , biochemistry , in vitro
Retinol (Vitamin A), is a well-known supplement for the repair of the wound, cell growth, and good vision. Through its implication in immune function is well established, further its recommendation as a supplement during the prognosis from infection along with antibacterial substances is still uncertain. Our study aims at understanding its anti-inflammatory effect on some known inflammatory markers, blood from a Healthy donor was collected for isolation of PBMCs and further induced with or without LPS (E. coli and Klebsiella). After 18 h of incubation, mRNA was isolated for qRT PCR quantification and studied for its effect. Retinol had good inhibitory action on genes under study such as IL6, TNFα, NFκB, SAA, TLR4, PNOC which are known to be pro-inflammatory in presence of LPS. Whereas its action on IL10 a well-known anti-inflammatory cytokine is contradictory to its suppressive action. Indicating its role as an anti-inflammatory supplement that can hold the expression of many mediators participating in inflammation and aid in controlling inflammation-led cytokine Strom.