Open AccessFrequency of Mutant p53 Among Acute Lymphoblastic Leukemia Patients in Basrah, Iraq
Author(s) -
WN Ibrahem,
HJ Hasony,
Jamila Hassan
Publication year - 2012
Publication title -
qatar medical journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.171
H-Index - 9
eISSN - 2227-0426
pISSN - 0253-8253
DOI - 10.5339/qmj.2012.1.13
Subject(s) - medicine , lymphoblastic leukemia , chromosomal translocation , antibody , leukemia , gastroenterology , gene , immunology , genetics , biology
A study was carried out to investigate the status of p53 proteins and their relation to the occurrence of TEL-AML-1 translocation in children with acute lymphoblastic leukemia (ALL) attending the oncology unit at Basrah maternity and children hospital during the period from May 2009 to April 2010. A total of 100 blood samples were collected from 40 newly diagnosed ALL cases, and 60 healthy children served as controls. Anti-p53 antibody was detected by an enzyme-linked immunosorbent assay (ELISA), and TEL-AML-1 fusion gene was determined by reversetranscriptase polymerase reaction (RT-PCR) on RNA extracted from fresh blood samples. The overall proportion of anti-p53 was 20% in the leukemic patients, whereas none of the healthy control group showed anti-p53 positivity. This difference was statistically significant (P 0.05) between TEL-AML-1 translocation and anti-p53. None of the standard risk patients with positive TEL-AML-1 fusion gene displayed the anti-p53 while 33.3% of TEL-AML-1 positive with high risk displayed the antip53 antibody.