Open AccessEfecto de n-acetilglucosamina sobre plaquetas
Author(s) -
Laura Mayoral,
María del Socorro Pina-Canseco,
Eduardo Pérez-Campos,
María Teresa Hernández-Huerta,
Edgar Zenten ́-Galindo,
Margarito Martínez Cruz
Publication year - 2017
Publication title -
ra rió guendaruyubi
Language(s) - English
Resource type - Journals
eISSN - 2594-0562
pISSN - 2594-0554
DOI - 10.53331/rar.v1i1.0873
Subject(s) - platelet , chemistry , calcium , phosphatidylserine , thrombin , glucosamine , platelet activation , antibody , biochemistry , immunology , phospholipid , medicine , membrane , organic chemistry
The treatment of platelets with various amino-sugars such as glucosamine, N-acetylglucosamine, N-acetyl neuraminic acid and others is capable of inducing: changes in shape, increased levels of free calcium, the exposure of phosphatidylserine on platelet surfaces, in addition to reducing the release of ATP and PF4 and inhibiting platelet aggregation. The objective of this study was to evaluate the effect of N-acetylglucosa- mine on the platelets of subjects with resistance to insulin compared with healthy subjects. The platelets (80,000 platelets/mL) of subjects with resistance to insulin were washed and then incu- bated with N-acetylglucosamine for 10 minutes and marked with the antibodies anti-PAC-1 and anti-CD62P bonded to fluorochromes and Ca2+ by means of a Fluo-3 union. Our results indicate that N-acetylglucosamine inhibits thrombin-in- duced intraplatelet calcium, the joining of antibodies anti-GPIIb/IIIa and anti-P-selectine.