Open AccessQPCR detection and quantification of ‘Candidatus phytoplasma solani’ in tomato with primers targeting CPN60 gene
Author(s) -
Irina Mitina,
Aighiuni Bahşiev,
Валентин Митин,
Irina Zamorzaeva
Publication year - 2021
Language(s) - English
Resource type - Conference proceedings
DOI - 10.53040/gppb7.2021.20
Subject(s) - phytoplasma , biology , candidatus , gene , virology , polymerase chain reaction , 16s ribosomal rna , genetics , restriction fragment length polymorphism
Tomato is one of the most economically important crops in Republic of Moldova. However, it is affected by a number of pathogens. One of the wide spread diseases is stolbur caused by the infection agent ‘Candidatus Phytoplasma solani’. Accurate diagnostics of the disease at an early stage is essential for successful control of the disease. In this work, we describe detection and quantification of ‘Candida-tus Phytoplasma solani’ in tomato by real-time PCR, as well as suitability of the method for assessing resistance of different tomato varieties to Phytoplasma infection.