Open AccessStudy of the influence of negative temperatures on biomass accumulation and cell viability of callus and cell aggregates of Rhodiola rosea L.
Author(s) -
Tatiana Călugăru-Spătaru
Publication year - 2021
Language(s) - English
Resource type - Conference proceedings
DOI - 10.53040/gppb7.2021.07
Subject(s) - callus , rhodiola rosea , salidroside , viability assay , botany , rhodiola , cell , chemistry , microbiology and biotechnology , biology , biophysics , biochemistry , chromatography
As a result of determining the resistance to the action of different negative temperatures of callus cells and cellular aggregates of Rhodiola rosea, it was shown that after exposure of callus to -8oC, only 52% of the cells survived. In the case of exposing the experimental variant of R. rosea cell aggregates to -8oC, the value of cell viability was 68%. This suggests that the frost tolerance of cell aggregates is higher than that of callus cells, which indicates that the stress factor to be tolerated is higher, the lower the de-gree of organization of the biological system.