Open AccessSelection of glufosinate-resistant cotton lines (Gossypium hirsutum L.) among bar transgenic lines
Author(s) -
Nha Thanh Nguyen
Publication year - 2020
Publication title -
the journal of agriculture and development/the journal of agriculture and develoment/nông nghiệp và phát triển
Language(s) - English
Resource type - Journals
eISSN - 2615-949X
pISSN - 2615-9503
DOI - 10.52997/jad.9.05.2020
Subject(s) - glufosinate , transgene , biology , gossypium hirsutum , southern blot , genetically modified crops , gene , selection (genetic algorithm) , genetics , botany , microbiology and biotechnology , glyphosate , artificial intelligence , computer science
Basta-herbicide was tested at a concentration of 0.6 kg ai./ha for confirming resistance of 116 bar transgenic T1 lines; many lines with tolerance were obtained. Evaluation of selected lines using PCR, the integration and expression of transgenes in genome of transgenic plants was determined by southern blot and northern blot techniques. The combination of molecular and biological assessments resulted in the selection of 5 lines, i.e., B1, B6, B9, B18, and BF17 contained 01 target-gene copy which expressed transcription activities and showed uniform growth and best tolerance to glufosinate. Two T2 transgenic cotton lines, i.e., B9 and BF17, carried one copy of the gene which transmitted to the next generation according to the Mendel's rules of inheritance. These transgenic lines were highly resistant to Basta herbicide at a concentration of 0.6 kg ai./ha and had no difference in botanical characteristics and disease resistance in comparison with original non-transgenic Coker310 cotton plant