Open AccessAdjustment of the formula of local surface water quality assessment
Author(s) -
Lan T. Q. Tran
Publication year - 2018
Publication title -
the journal of agriculture and development/the journal of agriculture and develoment/nông nghiệp và phát triển
Language(s) - English
Resource type - Journals
eISSN - 2615-949X
pISSN - 2615-9503
DOI - 10.52997/jad.8.05.2018
Subject(s) - quality (philosophy) , environmental science , water quality , surface water , business , environmental engineering , philosophy , epistemology , biology , ecology
Separation and identification of the active pharmacological compounds from some medicinal plants (Citrus aurantifolia, Rhodomyrtus tomentosa, Camellia sinensis) by Thin Layer Chromatography (TLC) showed the yields of crude extracts obtained from Citrus aurantifolia, Rhodomyrtus tomentosa and Camellia sinensis leaves at 1.5%, 5.62% and 10.4% respectively. Toluene: ethyl acetate (93:7) (v:v) solvent was suitable for the separation of active compounds in crude extract of Citrus aurantifolia, Rhodomyrtus tomentosa leaves while chloroform: ethylacetate: formic acid (5:4:1) (v:v:v) solvent was suitable for the extraction of active compounds in crude extract from Camellia sinensis leaves. Furthermore, the results showed that the number of compounds in extract from Citrus aurantifolia were likely affected by the vacuum evaporator effects. The TLC fingerprints of all three medicinal plants had the same visual ability when the fingerprint detected by UV (λ = 254 nm) and the reagent containing 0.1 g vanillin in 28 mL of methanol: 1 mL of sulfuric acid. In brief, the three active compounds including citral (Citrus aurantifolia), rhdomyrtone (Rhodomyrtus tomentosa) and catechin hydrate (Camellia sinensis) contained in the extract of 3 medicinal herbs had the limited detection (LOD) at 195 ng/spot, 321.5 ng/spot and 625 ng/spot, respectively.