Open AccessGenetic characteristics in four farmed tilapia (Oreochromis niloticus) populations evaluated by microsatellite markers
Author(s) -
Ha Thi Thanh Tran
Publication year - 2019
Publication title -
the journal of agriculture and development/the journal of agriculture and develoment/nông nghiệp và phát triển
Language(s) - English
Resource type - Journals
eISSN - 2615-949X
pISSN - 2615-9503
DOI - 10.52997/jad.7.05.2019
Subject(s) - oreochromis , biology , microsatellite , nile tilapia , genetic diversity , allele , loss of heterozygosity , genetics , genotype , analysis of molecular variance , population , genetic variation , veterinary medicine , zoology , gene , fish <actinopterygii> , fishery , demography , medicine , sociology
In this study, genetic characteristics of four tilapia strains including NOVIT4, 13th GIFT generation, China and Philippine were investigated by analysis the genotype at 6 microsatellite loci. It was found that all loci showed high polymorphism with 51 identified alleles, in which GM139 and UNH995 loci were the highest polymorphism with the appearance of 14 and 11 alleles, respectively. Deficiency of expected heterozygosity occurred in all tilapia strains, the Ho value of which was lower than the He one. Besides, 17/24 loci were significantly different from the Hardy-Weinberg principle (P < 0,05). The Fst ranging from 0.012 to 0.025 indicated that the genetic difference among populations was small. However, the analysis of molecular variance (AMOVA) showed that the genetic diversity at the molecular level among individuals within the same population and among individuals were high, corresponding to 36.69% and 61%, respectively. The findings of this study are useful in term of giving the genetic information of four Nile tilapia strains for the selective breeding program.