Open AccessOptimization of total phenolic extraction of Chromolaena odorata leaf for antifungal activity against plant pathogens
Author(s) -
Biet V. Huynh
Publication year - 2019
Publication title -
the journal of agriculture and development/the journal of agriculture and develoment/nông nghiệp và phát triển
Language(s) - English
Resource type - Journals
eISSN - 2615-949X
pISSN - 2615-9503
DOI - 10.52997/jad.6.06.2019
Subject(s) - chromolaena odorata , extraction (chemistry) , fusarium oxysporum , phytophthora capsici , antifungal , phenols , chemistry , response surface methodology , fusarium , food science , botany , biology , phytophthora , chromatography , organic chemistry , microbiology and biotechnology , weed
Phenolic compounds are secondary metabolites mainly responsible for different pharmacological activities of the plant extracts. In this study, microwave-assisted extraction (MAE) variables were optimized for the extraction of the phenolic compounds from Chromolaena odorata (C. odorata) by using a central composite design (CCD) of response surface methodology (RSM). The optimized conditions were at 62oC for 3 min with solvent to feed ratio of 14:1 (mL/g) and ethanol concentration of 50% (v/v). The highest yield of total phenolic compounds was 75.76 mgGAE/g. Ethanol extract clearly showed antifungal activity through antifungal index. The antifungal effect Phytophthora capsici, Fusarium oxysporum and Colletotrichum siamese were 79.07%, 78.66% and 78.42%, in 0.5% of ethanol extract, respectively