Open AccessSynthesis and transfer of RNAi construct for potential knock-down of gene expression of root-knot nematode (Meloidogyne graminicola) to rice (Oryza sativa L.)
Author(s) -
Phong Van Nguyen
Publication year - 2020
Publication title -
the journal of agriculture and development/the journal of agriculture and develoment/nông nghiệp và phát triển
Language(s) - English
Resource type - Journals
eISSN - 2615-949X
pISSN - 2615-9503
DOI - 10.52997/jad.5.04.2020
Subject(s) - biology , effector , root knot nematode , oryza sativa , gene silencing , nematode , rna interference , gene , rna silencing , gene expression , genetics , microbiology and biotechnology , rna , ecology
Effectors play key roles in the parasitism of the plant-parasitic nematode. Silencing the effector-coding genes was applied to study the function and role of nematode effectors. In this study, the Mgra16281 gene (ID: MK322955.1) encoding an effector with the unknown function was cloned from the rice root-knot nematode Meloidogyne graminicola isolated in Long An province. To knock-down the expression of this gene, an artificial microRNA was synthesized based on the Osa-MIR528 precursor and inserted into an expression vector. This microRNA can be expressed in rice to investigate the function of MGRA16281 of root-knot nematode via host-induced gene silencing approach (HIGS).