Open AccessPartial purification and biochemical characterization of amylase from Aeromonas caviaeNK1isolated from Industrial waste of India
Author(s) -
Kalyani Khanra,
Indranil Choudhuri,
Sudipta Panja,
Nandan Bhattacharyya
Publication year - 2016
Publication title -
journal of biology and life science
Language(s) - English
Resource type - Journals
ISSN - 2157-6076
DOI - 10.5296/jbls.v7i1.8361
Subject(s) - chemistry , nuclear chemistry , amylase , starch , enzyme , biochemistry
A partial purification and biochemical characterization of theamylase from Aeromonascaviae NK1 were carried out in this study. The extracellular extract was concentrated using ammonium sulfate precipitation and optimum operational conditions for the enzyme activity from the strain were evaluated. The optimum pH and temperature were observed 11.5 and37 0 C respectively. Ca 2+ , Mn 2+ , Mg 2+ , Zn 2+ , Pb 2+ , Co 2+ were found to have effect on amylase activity.Furthermore, the analysis of kinetic showed that the enzyme has of 2.4mg/mL and max of 21853.0mol/min/mg for starch. The results indicate that the enzyme reflects their potentiality towards industrial utilization.