Open AccessGenomic DNA Isolation and Purification of Two Endemic Medicinal Plants (Pterocarpus Santalinus Linn.F & Pimpinella Tirupatiensis Bal&Subr) of Seshachalam Hills, Tirumala.
Author(s) -
S. Vipranarayana,
T. N. V. K. V. Prasad,
A. Rajinikanth,
T. Damodharam
Publication year - 2012
Publication title -
journal of biology and life science
Language(s) - English
Resource type - Journals
ISSN - 2157-6076
DOI - 10.5296/jbls.v4i1.1618
Subject(s) - genomic dna , dna extraction , traditional medicine , biology , medicinal plants , isolation (microbiology) , chromatography , dna , botany , chemistry , polymerase chain reaction , microbiology and biotechnology , biochemistry , medicine , gene
Ptercarpus santalinus is an important endemic medicinal plant with high medicinal values that interfere with DNA extraction procedures and qualitative, quantitative agaros gel electrophoresis. An effective and low-cost protocol for isolating genomic DNA from the roots of Pterocarpus santilinus and Pimpinella tirupatiensis was described in this paper. It involved a modified CTAB method with distilled water pretreating samples. The A260/A280 absorbance ratio of extracted DNA was found to be free from polysaccharide, poly-phenols and tannins contaminants ranged from 2.2 to 2.8 within the high level of purity.