Open AccessDisinfectants are widely used to get rid of microorganisms whether in hospitals, health centers or for normal domestic use. Some suggested that when disinfectants are incorrectly diluted the disinfectant might promote the growth of antibiotic-resistant bacteria, therefore, in this study pathogenic bacterium (Pseudomonas aeruginosa), isolated from patient with urinary tract infection, treated with two locally popular disinfectants (Claradone and Sarttol). Results showed that the bacterial growth was affected by both disinfectants. The lowest concentration of Claradone that inhibit the growth of this bacterium is considered as the minimum inhibitory concentration (MIC), this was 30%, while the lowest effecting concentration of Sarttol was 3%. A number of survival colonies after treated with high concentration of Calarodone and Sarttol were investigated for their susceptibility to antibiotics, using standard disc diffusion method. Results indicated that these colonies of P. aeruginosa resisted antibiotics they were sensitive to before treatment. So it can be concluded that using Claradone and sarttol can make the pathogenic bacterium (P. aeruginosa) resist some antibiotics.