Open AccessIsolation and Identification of Catechin by a New Method from Food Efficiency Stimulating Plant Alhagi camelorum
Author(s) -
Abdul Hafeez Laghari,
Shahabuddin Memon,
Aisha Nelofar,
Khalid M. Khan
Publication year - 2017
Publication title -
pakistan journal of scientific and industrial research. series a: physical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.136
H-Index - 4
eISSN - 2223-2559
pISSN - 2221-6413
DOI - 10.52763/pjsir.phys.sci.60.2.2017.59.66
Subject(s) - catechin , chemistry , chromatography , antioxidant , antioxidant capacity , linear correlation , food science , polyphenol , biochemistry , mathematics , statistics
The present article describes a new developed method for the simultaneous determination of(+)-catechin (1) and (-)-epicatechin (2), separated via HPLC. The method has been validated and appliedon the real samples and 1 has been detected in both aerial and root parts of Alhagi camelorum without anyambiguity of fake positive or negative presence of 1 or 2 by virtue of dual detection system of UV andMass Spectrometry. The antioxidant capacity was also investigated and a linear correlation has been noticedbetween the antioxidant capacity and the catechin amount in A. camelorum extracts