Open AccessEffect of Reconstitution Solvents and Containers on Kinetics and Safety of Cephradine Neutralised with L-Arginine
Author(s) -
Aman U. Khan,
Javeid Iqbal,
Saif-ur-Rehman Khattak,
Najam-us Saquib,
Muhammad Saleem Qazi
Publication year - 2015
Publication title -
pakistan journal of scientific and industrial research. series a: physical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.136
H-Index - 4
eISSN - 2223-2559
pISSN - 2221-6413
DOI - 10.52763/pjsir.phys.sci.58.3.2015.136.141
Subject(s) - chemistry , kinetics , nuclear chemistry , degradation (telecommunications) , reaction rate constant , sodium , chloride , chromatography , organic chemistry , telecommunications , physics , quantum mechanics , computer science
The effect of reconstitution solvents such as water, 0.5% metronidazole solution, 0.9% sodium chloride and 5% dextrose injections, have been investigated on the kinetics of degradation of cephradineneutralised with L-arginine contained in glass, polyvinylchloride (PVC) and polyethylene pthalate (PET) containers at 5, 15 and 30 oC. The analytical method described in USP-31 for the analysis of cephradineinjection was employed in this study and validation in respect of specificity, linearity, accuracy and precision was observed. The degradation of the compound showed first-order kinetics and the degradation rate constants kobs were found in the range of 1.84-3.07 ´ 10-3/h (r2 = 0.990-0.999) at 5 oC, 2.3-4.2 ´ 10-3/h (r2 = 0.993-0.999) at 15 oC and 7.18-9.97 ´ 10-3/h (r2 = 0.998-0.999) at 30 oC, respectively. Cephradineshowed maximum stability in dextrose solution followed by water, sodium chloride and metronidazole injections, however, linear effect of containers on degradation rate could not be established. The extended degradation did not change the kinetics of the reaction. The abnormal toxicity/ safety test on mice for the admixtures in different containers at various temperatures showed no abnormal toxicity.