Open AccessIn vitro Studies of the Utilization of Industrially Important Substrates by Lactobacillus sp. AAF-1 Isolated from Coconut Water
Author(s) -
Ayesha Siddiqui,
Ayisha Aman,
Syeda Areej Fatima,
Aliya Riaz,
Rashida Rahmat Zohra,
Suad Naheed
Publication year - 2022
Publication title -
pakistan journal of scientific and industrial research. series b: biological sciences
Language(s) - English
Resource type - Journals
eISSN - 2223-2567
pISSN - 2221-6421
DOI - 10.52763/pjsir.biol.sci.65.1.2022.62.66
Subject(s) - food science , cellulase , amylase , lactobacillus , chemistry , hydrolysis , protease , agar , lactose , cellulose , microbiology and biotechnology , biochemistry , enzyme , biology , bacteria , fermentation , genetics
Coconut water (Cocos nucifera L.) possesses natural hydrating qualities, functional health properties and various nutritional benefits. Lactobacillus species AAF-1 has been isolated and purified from coconut water. The isolated culture was screened for the production of extracellular secreted enzymes having industrial value. Growth of the Lactobacillus sp. AAF-1 on MRS agar containing respective substrates showed that the Lactobacillus sp. AAF-1 is the potential producer of amylase, protease, cellulase and beta galactosidase. Further cell free filtrates of the Lactobacillus sp. AAF-1 showed large zone of hydrolysis of starch, gelatin, cellulose and lactose that reflected the extracellular production of the above enzymes in culture medium at 50 °C. It has been observed that activities after 72 h of incubation in cell free filtrates of protease, amylase, cellulase and beta galactosidase as the zone of respective substrate hydrolysis measured as 10, 8, 6 and 10 mm respectively. Thus, Lactobacillus sp. AAF-1 could be the potential producer of the enzymes having industrial value.