Open AccessAntioxidant and Antidiabetic Potential of Saponin Fraction Isolated from Moringa oleifera Leaves
Author(s) -
Fatma Hussain,
Fatima Ikram
Publication year - 2020
Publication title -
pakistan journal of scientific and industrial research. series b: biological sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.117
H-Index - 5
eISSN - 2223-2567
pISSN - 2221-6421
DOI - 10.52763/pjsir.biol.sci.63.2.2020.86.92
Subject(s) - moringa , chemistry , dpph , saponin , antioxidant , flavonoid , fractionation , food science , polyphenol , traditional medicine , chromatography , biochemistry , medicine , alternative medicine , pathology
To assess total phenolic contents, total flavonoid contents (TPC, TFC), DPPH scavenging and antidiabetic (antiglycation and alpha amylase hindrance) activities of Moringa oleifera (MO) leaves, bio- assay guided fractionation was used with thin layer chromatography profile to isolate saponin fraction (SF). The TPC and TFC were 9.7-12.8 GAE and 5.3-9.3 CE (g/100 g dry weight), respectively and aqueous and methanol fractions were more effective. DPPH scavenging activity ranged 69.5-83.1%. SF displayed noteworthy antioxidant ability (p 0.198), nullifying its concentration dependent impact.