Improvement of Amylase Production by UV Mutagenesis of Aspergillus flavus FSS63 under Solid State Fermentation | Zendy

Yasser Bakri | Zendy; Samir Elkhouri | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Improvement of Amylase Production by UV Mutagenesis of Aspergillus flavus FSS63 under Solid State Fermentation

Author(s) -

Yasser Bakri,

Samir Elkhouri

Publication year - 2016

Publication title -

biological sciences - pjsir

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.117

H-Index - 5

eISSN - 2223-2567

pISSN - 2221-6421

DOI - 10.52763/pjsir.biol.sci.59.2.2016.80.84

Subject(s) - amylase , solid state fermentation , aspergillus flavus , food science , bran , fermentation , starch , spore , strain (injury) , maltose , incubation , agar plate , mutant , biology , chemistry , bacteria , microbiology and biotechnology , biochemistry , enzyme , sucrose , raw material , genetics , anatomy , gene , ecology

Enhancement of the amylase productivity by Aspergillus flavus was investigated. Spores ofstrain were exposed to ultraviolet (UVC) radiation and 10 different mutants were selected and isolatedfrom starch plate agar on the basis of the visible clearance zone around the colonies. The amylase productionby selected mutants was evaluated under solid state fermentation. One mutant of A. flavus FSS63UV8showed higher biosynthesis level of amylase (733 IU/g), which was 3.35 fold higher than that detectedin the parental strain. Physical parameters optimisation revealed that the optimum pH and temperature foramylase production obtained by mutant are 7.0 and 35°C, respectively. Among several tested agriculturalwastes , wheat bran was found to support the highest yield of amylase after 5 days of incubation. A. flavusFSS63UV8 strain proved to be a promising microorganism for a high amylase production in a simplemedium.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research