Open AccessAN OPTIMIZED GC-MS METHOD FOR AMPHETAMINES IDENTIFICATION
Author(s) -
Georgi Bonchev,
Snezha Zlateva,
Petko Marinov,
Ivaylo Vazharov
Publication year - 2017
Publication title -
journal of imab
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.225
H-Index - 2
ISSN - 1312-773X
DOI - 10.5272/jimab.2017232.1603
Subject(s) - identification (biology) , gas chromatography–mass spectrometry , chromatography , computer science , chemistry , mass spectrometry , biology , botany
Purpose: To develop a reliable method for qualitative determination of amphetamines in biological samples, that combines simplicity, sensitiveness and robustness. Optimization of the method should be carried out in order to meet the needs of forensic expertise as daily routine.\udMaterial/Methods: Analytical identification was done by menas of gas chromatography – mass spectrometry. Validation was carried out using certified analytical standards.\udResults: Sample preparation procedure has been simplified. Only common reagents are needed. Solid phase extraction (SPE) has been used for initial purification of biological samples. Additional derivatization (PFPA) has been shown to be of major importance. Limit of detection (LOD) in blood samples has been determined to be as low as 25 ng/mL for amphetamine (AMP) and 10 ng/mL for methamphetamine (MET). Results are given within 100 minutes