Open AccessIncrease of sister chromatid exchange and unscheduled synthesis of deoxyribonucleic acid by acrylonitrile in human lymphocytes in vitro.
Author(s) -
P Perocco,
G Pane,
Silvana Bolognesi,
Maria Zannotti
Publication year - 1982
Publication title -
scandinavian journal of work, environment and health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.621
H-Index - 103
eISSN - 1795-990X
pISSN - 0355-3140
DOI - 10.5271/sjweh.2464
Subject(s) - acrylonitrile , sister chromatid exchange , sister chromatids , in vitro , dna , thymidine , dna synthesis , chemistry , microbiology and biotechnology , biochemistry , nuclear chemistry , biology , organic chemistry , chromosome , copolymer , gene , polymer
The investigation has been carried out on cultures grown in the presence of 5 x 10(-1) - 5 x 10(-5) M acrylonitrile with or without a rat liver metabolizing system (S-9 mix). The results obtained showed that acrylonitrile was toxic starting from the 5 x 10(-3) M concentration, caused a significant increase in the sister chromatid exchange (SCE) frequency in comparison with the controls (p less than or equal to 0.001) when the concentration was 5 x 10(-4) M, and elicited reparative deoxyribonucleic acid (DNA) synthesis, determined by tritiated thymidine uptake, particularly when the concentration was 5 x 10(-1) M. These effects were observed in lymphocytes of different donors and after drug activation by the S-9 mix metabolizing system.