Increase of sister chromatid exchange and unscheduled synthesis of deoxyribonucleic acid by acrylonitrile in human lymphocytes in vitro. | Zendy

Paolo Perocco | Zendy; G Pane | Zendy; Silvana Bolognesi | Zendy; Maria Zannotti | Zendy

Open Access

Increase of sister chromatid exchange and unscheduled synthesis of deoxyribonucleic acid by acrylonitrile in human lymphocytes in vitro.

Author(s) -

Paolo Perocco,

G Pane,

Silvana Bolognesi,

Maria Zannotti

Publication year - 1982

Publication title -

scandinavian journal of work environment and health

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.621

H-Index - 103

eISSN - 1795-990X

pISSN - 0355-3140

DOI - 10.5271/sjweh.2464

Subject(s) - acrylonitrile , sister chromatid exchange , sister chromatids , in vitro , dna , thymidine , dna synthesis , chemistry , microbiology and biotechnology , biochemistry , nuclear chemistry , biology , organic chemistry , chromosome , copolymer , gene , polymer

The investigation has been carried out on cultures grown in the presence of 5 x 10(-1) - 5 x 10(-5) M acrylonitrile with or without a rat liver metabolizing system (S-9 mix). The results obtained showed that acrylonitrile was toxic starting from the 5 x 10(-3) M concentration, caused a significant increase in the sister chromatid exchange (SCE) frequency in comparison with the controls (p less than or equal to 0.001) when the concentration was 5 x 10(-4) M, and elicited reparative deoxyribonucleic acid (DNA) synthesis, determined by tritiated thymidine uptake, particularly when the concentration was 5 x 10(-1) M. These effects were observed in lymphocytes of different donors and after drug activation by the S-9 mix metabolizing system.

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