Open AccessSampling of high concentrations of airborne fungi.
Author(s) -
Göran Blomquist,
Gunnar Ström,
Stromquist Lh
Publication year - 1984
Publication title -
scandinavian journal of work, environment and health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.621
H-Index - 103
eISSN - 1795-990X
pISSN - 0355-3140
DOI - 10.5271/sjweh.2357
Subject(s) - petri dish , agar , spore , agar plate , mold , homogenization (climate) , contamination , agar gel , sampling (signal processing) , biology , chromatography , aerobiology , environmental science , chemistry , botany , microbiology and biotechnology , ecology , bacteria , computer science , biodiversity , genetics , filter (signal processing) , computer vision
The paper discusses a method developed for determining airborne fungi particles in environments highly contaminated with mold fungi. The collection of airborne fungi was performed with two slit samplers. These sampling devices were found to give the highest values out of the three different types tested simultaneously. After spores had been collected with the slit samplers, the collection medium, an agar gel, was removed from the petri dishes and homogenized in a sterile 0.9% sodium chloride solution. The homogenate was diluted stepwise and spread on agar plates prior to the cultivation and the determination of viable counts. When the homogenization procedure was tested on samples collected from three different work environments, no increase or decrease in the number of colony-forming units could be detected. Storage of the homogenate at 2 degrees C over 9 d did not increase the number of viable fungal colonies.