Open AccessCRISPR/Cas9-based Antibody Production in Plants
Author(s) -
Bareera Zahoor,
Ummara Waheed,
S. A. Saeed,
Fatima Gulzar,
Hira Tasleem,
Muhammad Usman,
Zulqurnain Khan
Publication year - 2020
Publication title -
pakistan journal of biochemistry and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 2788-4503
pISSN - 2707-5494
DOI - 10.52700/pjbb.v1i2.19
Subject(s) - crispr , agroinfiltration , antibody , cas9 , plasmid , biology , computational biology , computer science , gene expression , gene , immunology , biochemistry
Nowadays demand of antibody production is increased to cure different diseases including diabetes, hepatitis and cancer. For that different types of systems are used for the expression of antibody production. But these were not improved the antibody production. Plant cells have several benefits in comparison with other eukaryotic cells if it is considered as eukaryotic expression system. As compared to the human cell or other microorganisms, the plant cell is safe and decrease the contamination of antibody production. In addition, plants perform proper post-translational modification as a eukaryotic expression system. But recently, transient expression system is used due to the safe and improve the quality and quantity of antibody production. In transient expression system agroinfiltration method are mostly used. The main issue in antibody production is purification. Because in downstream process antibody is degraded due to the physical and chemical stresses. These issues can be solved with the help of CRISPR/Cas9. Plant antibody can be tagged with the help of CRISPR/Cas9. This review encompasses the applications of CRISPR technology for producing plant-based antibodies.