Open AccessPotential uses of Lipopolysaccharide and Exopolysaccharide Isolated from Agrobacterium spp.
Author(s) -
Sadia Tahir,
Shahida Hasnain
Publication year - 2020
Publication title -
the journal of microbiology and molecular genetics
Language(s) - English
Resource type - Journals
eISSN - 2788-8312
pISSN - 2707-6431
DOI - 10.52700/jmmg.v1i1.6
Subject(s) - agrobacterium , biology , gram , bacteria , biological pest control , microbiology and biotechnology , lipopolysaccharide , permethrin , horticulture , pesticide , agronomy , transformation (genetics) , biochemistry , genetics , gene , endocrinology
A gram-negative bacterium, Agrobacterium, synthesizes lipopolysaccharide and exopolysaccharide, both indispensable in establishing complex interactions with plant hosts. These polysaccharides have biocontrol applications. This study intended to determine: 1) LPS potential as insecticide; 2) EPS production capacity. LPSs obtained from Agrobacterium isolates were tested for insecticidal activity against test insects, damaging stored grains. Agrobacterial LPS elicits insect mortalities comparable to Permethrin (standard insecticide). Typical strains (At2, At3 At5, At6) incited 80% mortality of tested insects. EPS yield was quantified at regular intervals under varying physical parameters. Maximum EPS yield of 12.25 g/L was recorded for strain At1. Antibacterial activity of all isolates (before and after UV light exposure) was tested against Gram-positive and Gram-negative reference strains. Agrobacteria exposed to UV light exhibited antibacterial activity against Gram-positive reference strains. Therefore, agrobacterial LPS can be future candidate for biocontrol of insect pests in grain stocks. Moreover, agrobacteria can not only be prospective candidates for industrial scale EPS production but can also potentially help in treating infections by antibiotic resistant Gram-positive bacteria. Hence, same native isolates carry numerous benefits for the community.