Open AccessLipid spectrum in patients with pulmonary tuberculosis
Author(s) -
Dmitriy Ryasenskiy,
AUTHOR_ID,
Alexander Aseev,
Akhmed Elgali,
AUTHOR_ID,
AUTHOR_ID
Publication year - 2022
Publication title -
buletinul academiei de ştiinţe a moldovei: ştiinţe medicale
Language(s) - English
Resource type - Journals
ISSN - 1857-0011
DOI - 10.52692/1857-0011.2021.3-71.15
Subject(s) - lysophosphatidylethanolamine , lysophosphatidylcholine , phosphatidylethanolamine , sphingomyelin , phosphatidylserine , chemistry , phosphatidylcholine , phospholipid , chromatography , phosphatidylinositol , membrane , biochemistry , lipidomics , kinase
The evaluation of the lipid spectrum of the blood plasma and immunocyte membranes is important for timely diagnostic and correction of pathological changes revealed at the cytochemical level. The identification of lipid fractions using high-performance liquid chromatography (HPLC) allows for separate detection of minor fractions of lysophospholipids (lysophosphatidylcholine and lysophosphatidylethanolamine). The study was performed using a photometric sensor and reverse-phase column C18. The following fractions were obtained: lysophosphatidylcholine, lysophosphatidylethanolamine, sphingomyelin, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, and phosphatidylserine. The ratio of these fractions is a sensitive marker of pathological shifts in the lipid spectrum both in the blood plasma and immunocyte membranes