Open AccessOmega-3 and Vitamin E Supplementation Effect to Rattus Norvegicus Placental Apoptosis Marker: Pre-Eclampsia Model
Author(s) -
Yesi Gusti,
Amir AlmasiHashiani,
Gusti Revilla
Publication year - 2021
Publication title -
international journal of science and healthcare research
Language(s) - English
Resource type - Journals
ISSN - 2455-7587
DOI - 10.52403/ijshr.20211037
Subject(s) - oxidative stress , vitamin d and neurology , apoptosis , vitamin e , proinflammatory cytokine , analysis of variance , eclampsia , omega 3 fatty acid , vitamin , endocrinology , medicine , pregnancy , chemistry , vitamin c , andrology , polyunsaturated fatty acid , biology , antioxidant , fatty acid , docosahexaenoic acid , biochemistry , inflammation , genetics
Background: Pre-eclampsia has been associated with the increased of placental apoptosis caused by oxidative stress. Omega-3 fatty acids and vitamin E had beneficial function to maintain cell membranes, prevent oxidative stress and inhibit the production of proinflammatory cytokines. The purpose of the study was to determine the effect of omega-3 and vitamin E supplement to BCL-2 and BAX on PE rats model.Methods: This research has been worked out at animal house and Biomedical Laboratory of the Medical Faculty Andalas University. The design of this research was experimental study with post test only control group design. There were 35 pregnant rats were divided into 5 groups. Group K- without treatment, group K+ was given L-NAME, group P1 was given L-NAME + omega-3, group P2 was given L-NAME + vitamin E and group P3 was given L-NAME + omega-3 + vitamin E. The L-NAME dose was 50mg/kg/day, At the 19th day of gestation, the pregnancy was terminated and the placental tissue was collected for examining BCL-2 and BAX levels by using ELISA kit. The data were analyzed by one way ANOVA and post hoc test LSD.Results: The mean levels of BCL-2 in each group were K- =62.49 ng/mg, K+ =50.64 ng/mg, P1 =58.79 ng/mg, P2 =53.70 ng/mg and P3 =70.10 ng/mg. The mean levels of BAX were K- =72.06 ng/mg, K+ =78.61 ng/mg, P1 =73.71 ng/mg, P2 =74.31 ng/mg and P3 =71.63 ng/mg. The analysis results concluded that there were significant differences between omega-3 and vitamin E supplements on BCL-2 levels (p=0.000) and BAX levels (p=0.001).Conclusion: Omega-3 (DHA 120 mg, EPA 180 mg) and vitamin E (α-tocopherol 300 iu) suplement increased BCL-2 level and decreased BAX on pre-eclampsia rats model.Keywords: Omega-3, Vitamin E, BCL-2, BAX, Pre-eclampsia.