Open AccessComparison of Different Phenotypic Methods for Detection of Vancomycin Drug Resistance in Enterococcus Species
Author(s) -
Deepa Devhare,
S. Pol
Publication year - 2021
Publication title -
international journal of research and review
Language(s) - English
Resource type - Journals
eISSN - 2454-2237
pISSN - 2349-9788
DOI - 10.52403/ijrr.20210939
Subject(s) - vancomycin , enterococcus , drug resistance , enterococcus faecium , vancomycin resistant enterococcus , microbiology and biotechnology , medicine , biology , antibiotics , bacteria , staphylococcus aureus , genetics
Vancomycin resistant enterococci have emerged as an important cause of nosocomial infection worldwide. Vancomycin drug resistance needs to be detected accurately in all Enterococcus species in order to prevent its spread in health care setting. Present study was conducted to compare three different phenotypic methods for detection of vancomycin resistance in enterococci.Material and methods: Study was conducted in a tertiary care hospital over a period of one year. Enterococcus species isolated from clinical samples like urine, pus, blood and sterile body fluids were tested by three different methods namely disk diffusion, E-strip and Phoenix automated system for detection of vancomycin resistance.Results: 400 Enterococcus species were isolated from clinical samples. 19(4.8%) Enterococcus species were found to be vancomycin resistant and one (0.25%) strain was found to be intermediate resistant to vancomycin by all three methods resulting in 100% sensitivity and 100%specificity.Conclusion: Present study recommends vancomycin disk diffusion as screening and E-strip as good confirmatory tests in resource poor settings for detection of vancomycin drug resistance.Keywords: VRE, vancomycin, disk diffusion, E strip, Phoenix automated system..