Open AccessEarly Detection of Blood Borne Pathogens and its Antibiotic Susceptibility
Author(s) -
Uday Rajesh Titare,
Ravindra Ashok Sharma
Publication year - 2022
Publication title -
international journal of health sciences and research
Language(s) - English
Resource type - Journals
ISSN - 2249-9571
DOI - 10.52403/ijhsr.20220428
Subject(s) - macconkey agar , staphylococcus epidermidis , medicine , chocolate agar , microbiology and biotechnology , antibiotics , agar plate , klebsiella pneumonia , staphylococcus aureus , bacterial growth , blood culture , agar , staphylococcus , bacteria , biology , genetics
In this study the evaluation of the new blood culture technique to improve rapid detection for the presence of bacteria in blood by using Tetrazolium dye (TTC) was done. To compare and evaluate effective method with the help of MICs values obtained from improved method against standard method. The Blood samples were collected and processed by two different method i.e. conventional blood culturing method and improved method by using TTC dye. In conventional method the sample was inoculated in blood culture bottle and then plated on blood agar, MacConkey, and chocolate agar and was incubated for 24hrs. After incubation the growth observed was identified and reported. Along with this in Improved TTC dye method the blood was inoculated in tryptone soya broth incubated and then TTC dye was added in it and cherry red color development was considered as positive. In the present study, total 50 blood samples were processed of which 32 samples exhibited growth on media and 18 samples show no growth. From total positive growth samples, 9 were gram positive including Staphylococcus aureus, Staphylococcus haemolyticus and Staphylococcus epidermidis and remaining 23 were gram negative rods including E. coli, Pseudomonas aeruginosa and Klebsiella pneumonia. Overall, the average time required for growth by conventional method is 51 hours while that of TTC dye method is of 30 hours. In contrast to antibiotic susceptibility test, Microbroth dilution method was easy, reliable, affordable and quick result giving method as compare to standard antibiotic susceptibility test method.Key words: Bloodstream infections, Antibiotic susceptibility, Tetrazolium dye.